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goat anti human sod3 ec sod  (R&D Systems)


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    R&D Systems goat anti human sod3 ec sod
    Goat Anti Human Sod3 Ec Sod, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+human+sod3+ec+sod/Human+SOD3%2FEC-SOD+Antibody/pmc06295554-62-27-32
    Average 93 stars, based on 6 article reviews
    goat anti human sod3 ec sod - by Bioz Stars, 2026-10
    93/100 stars

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    Blocking Assay:

    Article Title: Lung-Specific Extracellular Superoxide Dismutase Improves Cognition of Adult Mice Exposed to Neonatal Hyperoxia
    Article Snippet: Protein samples were diluted in Laemmli buffer, separated by SDS-PAGE, and then transferred to polyvinylidene difluoride membranes (Pall Life Sciences, Port Washington, NY, United States). .. After blocking the membranes with 5% non-fat dry milk, they were incubated overnight with goat anti-mouse SOD3/EC-SOD (1:500 dilution; R & D Systems, Minneapolis, MN, United States), goat anti-human SOD3/EC-SOD (1:500 dilution; R & D Systems), or rabbit anti-mouse β-actin (1:1,000 dilution; Sigma, St. Louis, MO, United States) antibodies. .. The membranes were then incubated with the appropriate horseradish peroxidase-conjugated secondary antibody (anti-goat at 1:5,000 and anti-rabbit at 1:5,000; Southern Biotechnology) and immune complexes were visualized by enhanced chemiluminescence (ECL kit, GE Life Sciences, Piscataway, NJ, United States).

    Incubation:

    Article Title: Lung-Specific Extracellular Superoxide Dismutase Improves Cognition of Adult Mice Exposed to Neonatal Hyperoxia
    Article Snippet: Protein samples were diluted in Laemmli buffer, separated by SDS-PAGE, and then transferred to polyvinylidene difluoride membranes (Pall Life Sciences, Port Washington, NY, United States). .. After blocking the membranes with 5% non-fat dry milk, they were incubated overnight with goat anti-mouse SOD3/EC-SOD (1:500 dilution; R & D Systems, Minneapolis, MN, United States), goat anti-human SOD3/EC-SOD (1:500 dilution; R & D Systems), or rabbit anti-mouse β-actin (1:1,000 dilution; Sigma, St. Louis, MO, United States) antibodies. .. The membranes were then incubated with the appropriate horseradish peroxidase-conjugated secondary antibody (anti-goat at 1:5,000 and anti-rabbit at 1:5,000; Southern Biotechnology) and immune complexes were visualized by enhanced chemiluminescence (ECL kit, GE Life Sciences, Piscataway, NJ, United States).



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